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Overview
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Product name
Anti-Lrp2 / Megalin antibody
See all Lrp2 / Megalin primary antibodies -
Description
Rabbit polyclonal to Lrp2 / Megalin
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Host species
Rabbit
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Tested applications
Suitable for: IHC-Pmore details
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Species reactivity
Reacts with: Mouse, Rat, Human, Monkey
Predicted to work with: Xenopus laevis
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Immunogen
Synthetic peptide corresponding to Human Lrp2/ Megalin aa 4600 to the C-terminus (C terminal).
(Peptide available asab101417) -
Positive control
- IHC-P: Mouse kidney and human kidney carcinoma tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
See Also#102 Increased expression of carbonic anhydrase 4 in megalin deficiencyMegalin knockout reduces SGLT2 expression and sensitizes to Western diet-induced kidney injury#1284 Proteinuria causes excessive proliferation and senescence in proximal tubular cells via receptor-mediated endocytosisRecombinant Anti-Lrp2 / Megalin antibody [RM1150] (ab317333)
Properties
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Form
Liquid
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBSBatches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
- Concentration information loading...
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Purity
Immunogen affinity purified
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Clonality
Polyclonal
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Isotype
IgG
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Research areas
- Cardiovascular
- Lipids / Lipoproteins
- Fatty Acids
- Binding proteins
- Stem Cells
- Lineage Markers
- Endoderm
- Stem Cells
- Signaling Pathways
- Hedgehog
- Surface Molecules
- Developmental Biology
- Lineage specification
- Endoderm
- Cancer
- Cancer Metabolism
- Metabolic signaling pathway
- Hormone biosynthesis
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
- Metabolism
- Pathways and Processes
- Endocrine metabolism
- Hormone biosynthesis
- Metabolism
- Types of disease
- Obesity
- Metabolism
- Types of disease
- Cancer
- Metabolism
- Pathways and Processes
- Redox metabolism
- Fatty acid oxidation
Associated products
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Compatible Secondaries
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Isotype control
- Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (9) | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| Notes |
|---|
| IHC-P |
Target
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Function
Acts together with cubilin to mediate HDL endocytosis (By similarity). May participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release.
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Tissue specificity
Absorptive epithelia, including renal proximal tubules.
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Involvement in disease
Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS) [MIM:222448]; also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity.
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Sequence similarities
Belongs to the LDLR family.
Contains 17 EGF-like domains.
Contains 36 LDL-receptor class A domains.
Contains 37 LDL-receptor class B repeats. -
Cellular localization
Membrane. Membrane > coated pit.
- Information by UniProt
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Database links
- Entrez Gene: 4036 Human
- Entrez Gene: 14725 Mouse
- Entrez Gene: 29216 Rat
- Omim: 600073 Human
- SwissProt: P98164 Human
- SwissProt: A2ARV4 Mouse
- SwissProt: P98158 Rat
- Unigene: 657729 Human
see all
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Alternative names
- Calcium sensor protein antibody
- DBS antibody
- Glycoprotein 330 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody (ab76969)
IHC image of Lrp2 / Megalin staining in normal Mouse Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76969, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody (ab76969)
IHC image ofLrp2/Megalin antibodystaining in a section of formalin-fixed paraffin-embedded human kidney renal carcinoma* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab76969, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody (ab76969)Image courtesy of an anonymous Abreview.
ab76969 staining Lrp2 / Megalin in monkey kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate pH 6.0. Samples were then incubated with ab76969 at 1µg/ml for 1 hour with BSA in TBS. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal.See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody (ab76969)This image is courtesy of an anonymous Abreview.
ab76969 stainingLrp2 /Megalinin mouse kidney tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde,blocked for 60 minutes at 25°C and antigen retrieval was by heat mediation. The sample was incubated with primary antibody (1/1000) at 25°C for 1 hour. An Alexa Fluor® 488-conjugateddonkey rabbit polyclonal (1/1200) was used as the secondary antibody.
See Abreview
Datasheets and documents
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SDS download
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Datasheet download
Download
References (41)
Publishing research using ab76969? Please let us know so that we can cite the reference in this datasheet.
ab76969 has been referenced in 41 publications.
- Yamaguchi Y et al. Hyperthyroidism exacerbates ischemic reperfusion injury in the kidney. Endocr J 69:263-272 (2022). PubMed: 34629337
- Silva-Aguiar RP et al. Albumin Expands Albumin Reabsorption Capacity in Proximal Tubule Epithelial Cells through a Positive Feedback Loop between AKT and Megalin. Int J Mol Sci 23:N/A (2022). PubMed: 35055044
- Ngo TTT et al. Functional differentiation and scalable production of renal proximal tubular epithelial cells from human pluripotent stem cells in a dynamic culture system. Cell Prolif 55:e13190 (2022). PubMed: 35102634
- Shen TH et al. Snapshots of nascent RNA reveal cell- and stimulus-specific responses to acute kidney injury. JCI Insight 7:N/A (2022). PubMed: 35230973
- Nahaboo W et al. Keratin filaments mediate the expansion of extra-embryonic membranes in the post-gastrulation mouse embryo. EMBO J 41:e108747 (2022). PubMed: 35266581
View all Publications for this product
Customer reviews and Q&As
